吴缅教授

文章出处: 作者: 发布时间: 2015-02-13 访问次数: 655

吴缅,教授,  1982年本科毕业于南京师范大学生物系,1984年获美国哥伦比亚大学生物化学硕士,1988年获哥伦比亚大学分子生物学博士,1988-1990美国哈佛大学细胞发育系的博士后。1991-1995新加坡大学生物学系担任助理教授。 1996-1998新加坡中央医院神经内科基因治疗组高级研究员。2004-2006新加波南洋理工大学生物系副教授。2000年至今,中国科学技术大学生命科学学院“百人计划”特聘教授,博士生导师,中科院百人计划引进人才。承担国家973项目4项,任课题组长。2006年以来,连续三次承担国家基金委重点项目,并承担中科院先导项目子课题负责人。多年来,在肿瘤细胞凋亡/生存的信号转导及分子机制,非编码RNA调控肿瘤发生发展的分子机理,p53与细胞代谢及诱导性多能干细胞iPS调控的分子机制方面做了开创性的工作。在SCI杂志上共发表了论文60多篇研究论文,被国际同行引用超过1500次。其中在国际上具有重要影响力的期刊,如Nature Cell BiologyMolecular Cell, Nature Communications,PNAS, EMBO Journal上作为通讯作者发表.目前担任Journal of Molecular and Cell Biology 副主编,Acta Biochimica etBiophysica Sinica 编委。是中科院天然免疫与慢性疾病重点实验室副主任,中科院细胞生物学国家重点实验室学术委员会委员,并担任中国细胞生物学学会第十届理事会理事。

研究方向

肿瘤发生发展的分子机理。

p53与细胞代谢

诱导性多能干细胞iPS调控的分子机制

获奖情况、专业学会会员

1.   2003年“挑战杯”全国大学生一等奖论文指导教师奖

2.   2003年“第七届复旦大学谈家桢基金生命科学九源奖学金”(一等奖)优秀指导教师奖

3.   2005年全国首届华侨华人专业人士杰出创业奖(全国100)

4.   2004年,2006年, 2009年,20114次获得中国科学院优秀研究生指导教师奖

5.   2007年,20122次获得全国百篇优秀论文提名奖

6.   2008年,2010年,20123次获得中国科学院朱李月华优秀教师奖

7.   2011 年中国科学技术大学杰出研究校长奖

在研经费

1.非编码RNA及线粒体代谢调控体细胞重编程 XDA01020104 中国科学院 2011.01-2015.12586.19万元 CX2070000058 子课题负责人;

2.干细胞编程与重编程中表观遗传密码识别和解读的分子机制和结构基础 2011CB966302 科技部 2011.01-2015.12137万元 课题骨干;

3p53家族蛋白及其相关microRNA调控细胞代谢的机制研究 20123402130006 教育部 2013.01-2015.1240万元 负责人

4Urdp1Drf1在细胞分化中作用机制的研究 31371388 国家自然科学基金委员会 2014.01-2017.1290万元 项目负责人;

5p53家族蛋白调控的细胞代谢在肿瘤发生发展中的作用及机制研究 81430065 国家自然科学基金 2015.01-2019.12320万元项目负责人;

管理工作及社会服务

1.   Journal of Molecular and Cell Biology Associate Editor

2.   Acta Biochimica etBiophysica Sinica编委

3.   World Journal of Translational Medicine编委

4.   中科院分子细胞生物学国家重点实验室学术委员会委员

5.   中国科学院天然免疫与慢性疾病重点实验室副主任

联系方式

办公室: 05513606264实验室: 0551-3607324

手机:13485679900

电子邮件:wumian@ustc.edu.cn

文章发表

(1)   Bo Chu, Liangwen Zhong, Shuang Dou, Jun Wang, Jianmin Li, Mingwei Wang,   Qinghua Shi, Yide Mei and Mian Wu. miRNA-181 regulates embryo implantation   in mice through targeting leukemia inhibitory factor. J Mol Cell Biol. (2015, in   press)

(2)   Fan Yang, Huafeng Zhang, Yide Mei, and Mian Wu. Reciprocal regulation of   HIF-1α and lincRNA-p21 modulates the Warburg effect. Mol Cell. 2014 Jan     9;53(1):88-100

(3)   Hu W, Jin L, Jiang CC, Long GV, Scolyer RA, Wu Q, Zhang XD, Mei Y, Wu M. AEBP1 upregulation confers acquired resistance to BRAF (V600E) inhibition in melanoma. Cell Death & Disease. 2013 Nov 7;4:e914. doi: 10.1038/cddis. 2013.441.

(4)   Y Zhu, Z Yao, Z Wu, Y Mei and M Wu. Role of tumor necrosis factor alpha-induced protein 1 in paclitaxel resistance. Oncogene 2014 Jun 19;33(25):3246-55.

(5)   Xingwu Wang, Xiaocheng Zhao, Ping Gao and Mian Wu. c-Myc modulates microRNA processing via transcriptional regulation of Drosha Scientific Reports 2013;3:1942. doi: 10.1038/srep01942

(6)   Huang X, Wu Z, Mei Y, Wu M. XIAP inhibits autophagy via XIAP-Mdm2-p53 signalling. EMBO J. 2013 Aug 14;32(16):2204-16

(7)   Wenjing Du, Peng Jiang, Anthony Mancuso, Aaron Stonestrom, Michael D. Brewer, Andy J. Minn, Tak W. Mak, Mian Wu* and Xiaolu Yang*. TAp73 enhances the pentose phosphate pathway and supports cell proliferation. Nat Cell Biol. 2013 Aug;15(8):991-1000

(8)   Wenyu Wang#, Bing Cheng#, Lin Miao, Yide Mei, and Mian Wu. Mutantp53-R273H gains new function in sustained activation of EGFR signaling via suppressing miR-27a expression Cell Death & Disease 2013 Apr 4;4:e574. doi: 10.1038/cddis.2013.97.

(9)   Xingwu Wang, Meng Zha, Xiaocheng Zhao, Peng Jiang, Wenjing Du, Andrew Y. H. Tam, Yide Mei, and Mian Wu. Siva1 inhibits p53 function by acting as an E3 ubiquitin ligase of ARF. Nature Communications. 2013;4:1551. doi: 10.1038/ncomms2533.

(10) Yang F, Miao L, Mei YD, Wu M. Retinoic acid-induced HOXA5 expression is co-regulated by HuR and miR-130a. Cellular Signaling 2013 Jun, 25(6):1476-1485

(11) Chuanchun Han, Hao Gu, Jiaxu Wang, Weiguang Lu, Yide Mei, and Mian Wu. Regulation of L-threonine dehydrogenase (TDH) in somatic cell reprogramming Stem Cells. January 2013; 25(1):144–149

(12) Xingwu Wang, Xiaocheng Zhao, Xiang Gao , Yide Mei, and Mian Wu. A new role of p53 in regulating lipid metabolism. J Mol Cell Biol. 2013 Apr;5(2):147-50.

(13) Han C, Jin L, Mei Y, Wu M. Endoplasmic reticulum stress inhibits cell cycle progression via induction of p27 in melanoma cells. Cellular Signaling. 2013 Jan;25(1):144-9.

(14) Jiaxu Wang, Qianqian He, Chuanchun Han, Hao Gu, Lei Jin, Qun Li, Yide Mei, and Mian Wu. p53-facilitated miR-199a-3p regulates somatic cell reprogramming. Stem Cells. 2012 Jul; 30(7):1405-13

(15) Mei Yede and Mian Wu. Multifaceted functions of Siva-1: more than an India God of Destruction. Protein & Cell. 2012 Feb,3(2):117-122

(16) Xie C, Wang W, Yang F, Wu M* and Mei Y*. RUVBL2 is a novel repressor of ARF transcription. FEBS Lett. 2012 (in press)

(17) Jin L, Hu WL, Jiang CC, Wang JX, Han CC, Chu P, Zhang LJ, Thorne RF, Wilmott J, Scolyer R, Hersey P, Zhang XD, and Wu M. MicroRNA-149*, a p53-responsive microRNA, functions as an oncogenic regulator in human melanoma. Proc Natl Acad Sci USA. 2011 Sep 20, vol. 108 no. 38 15840-15845

(18) Nan Li, Peng Jiang, Wenjing Du, Zhengsheng Wu, Cong Li, Mengran Qiao,Xiaolu Yang, and Mian Wu. Siva1 inhibits epithelial-mesenchymal transition and metastasis of tumor cells. Proc Natl Acad Sci USA.2011 108 (31) 12851-12856

(19) Jiang P, Du W, Wang X, Mancuso A, Gao X, Wu M*, Yang X*. p53 regulates biosynthesis through direct inactivation of glucose-6-phosphate dehydrogenase. Nat Cell Biol. 2011 Mar;13(3):310-6

(20) Xu Tian, Youjun Chen, Wanglai Hu, Mian Wu. E2F1 inhibits MDM2 expression in a p53-dependent manner. Cellular Signaling 2011 Jan;23(1):193-200. Epub 2010 Sep 17

(21) Guoxing Wang, Xiang Gao, Yun Huang, Zhan Yao, Qinghua Shi and Mian Wu. NPM/B23 inhibits Eg5-mediated microtubules depolymerization via inactivating its ATPase activity, J Biol Chem 2010 Jun 18;285(25):19060-7. Epub 2010 Apr 19.

(22) Zhan Yao, Shanshan Duan, Dezhi Hou, Wenyu Wang, Guoxing Wang, Yajing Liu, Longping Wen and Mian Wu. B23 acts as a nucleolar stress sensor and promotes cell survival via its dynamic interaction with hnRNPU and hnRNPA1. Oncogene, 2010 Mar 25;29(12):1821-34.

(23) Lin Miao, Zhiyin Song, Lei Jin, Y M Zhu, LP Wen and Mian Wu. ARF antagonizes the ability of Miz-1 to inhibit p53-mediated transactivation. Oncogene, 2010 Feb 4;29 (5):711-22.

(24) Wenjing Du, Peng Jiang, Nan Li , Yide Mei, Xingwu Wang, Longping Wen, Xiaolu Yang and Mian Wu. Suppression of p53 activity by Siva1 Cell Death & Differ 2009 Nov;16(11):1493-1504. (Featured article)

(25) Shanshan Duan, Zhan Yao, Youming Zhu, Guoxing Wang, Dezhi Hou, Longping Wen and Mian Wu. The Pirh2-Keratin 8/18 interaction modulates the cellular distribution of mitochondria and UV-induced apoptosis. Cell Death Differ 2009 Jun;16(6):826-37

(26) Wei Xie, Lei Jin, Yide Mei and Mian Wu. E2F1 represses beta-catenin/TCF activity by direct upregulation of Siah1 J Cell Mol Med 2009, Vol 13, Issue 8b, 1719-1727

(27) Mei Li, Shanshan Feng and Mian Wu. Multiple Roles for Nuclear Localization Signal (NLS, aa 442-472) of Receptor-Interacting Protein 3 (RIP3) Biochem Biophys Res Commun. 2008 Aug 8;372(4):850-5

(28) Yide Mei, Chongwei Xie, Wei Xie, Xu Tian, Mei Li and Mian Wu. Noxa/Mcl-1 balance regulates susceptibility of cells to Camptothecin-induced apoptosis, Neoplasia 2007 Oct;9(10):871-81.

(29) Feng S, Yang Y, Mei Y, Ma L, Zhu DE, Naser H, Castanares M, Wu M. Cleavage of RIP3 inactivates its caspase-independent apoptosis pathway by removal of kinase domain. Cell Signal. 2007 Jun 14;19(10):2056-2067.

(30) Shanshan Duan, Zhan Yao, Dezhi Hou, Zhengsheng Wu, Wei-guo Zhu and Mian Wu. Phosphorylation of Pirh2 by Calmodulin-dependent kinase II impairs its ability to ubiquitinate p53. EMBO J, 2007 Jul 11;26(13):3062-74.

(31) Yide Mei, Chongwei Xie, Wei Xie, Zhengsheng Wu, and Mian Wu. Siah-1Sa novel splice variant of Siah-1 (seven in absentia homolog), counteracts Siah-1-mediated downregulation of ?-catenin. Oncogene, 2007 Sep 20;26(43):6319-31.

(32) Zhan Yao, Shanshan Duan, Dezhi Hou, Klaus Heese and Mian Wu. Death effector domain DEDa, a self-cleaved product of Caspase-8/Mch5,translocates to the nucleus by binding to ERK1/2 and up-regulates procaspase-8 expression via a p53-dependent mechanism. EMBO J, 2007 261068-1080

(33) Jiang P, Du W, Wu M. p53 and Bad: remote strangers become close friends. Cell Res. 2007 Apr;17(4):283-5.

(34) Jiang P, Du W, Heese K, Wu M.The Bad Guy Cooperates with Good Cop p53: Bad Is Transcriptionally Up-Regulated by p53 and Forms a Bad/p53 Complex at the Mitochondria To Induce Apoptosis. Mol Cell Biol. 2006 Dec;26 (23):9071-9082

(35) Ma L, Huang Y, Song Z, Feng S, Tian X, Du W, Qiu X, Heese K, Wu M. Livin promotes Smac/DIABLO degradation by ubiquitin-proteasome pathway. Cell Death Differ. 2006 Dec;13 (12):2079-88

(36) Xie W, Jiang P, Miao L, Zhao Y, Zhimin Z, Qing L, Zhu WG, M Wu. Novel link between E2F1 and Smac/DIABLO: proapoptotic Smac/DIABLO is transcriptionally upregulated by E2F1. Nucleic Acids Res. 2006 Apr 14;34(7):2046-55..

(37) Feng S, Ma L, Yang Y, Wu M. Truncated RIP3 (tRIP3) acts upstream of FADD to induce apoptosis in the human hepatocellular carcinoma cell line QGY-7703. Biochem Biophys Res Commun. 2006 Sep 1;347(3):558-65.

(38) Zhu DE, Hoti N, Song Z, Jin L, Wu Z, Wu Q, Wu M. Suppression of tumor growth using a recombinant adenoviral vector carrying the dominant-negative mutant gene Survivin-D53A in a nude mice model.Cancer Gene Ther. 2006 Aug;13(8):762-70.

(39) Mei Y, Du W, Yang Y, Wu M. Puma*Mcl-1 interaction is not sufficient to prevent rapid degradation of Mcl-1. Oncogene. 2005 Nov 3;24 (48):7224-37.

(40) Johnson A, Gin P, Marbois BN, Hsieh EJ, Wu M. Barros MH, Clarke CF, Tzagoloff A COQ9, a new gene required for the biosynthesis of coenzyme Q in Saccharomyces cerevisiae. J Biol Chem. 2005 Sep 9;280 (36):31397-404.

(41) Song, ZY, Wu M. Identification of a novel nucleolar localization signal and a degradation signal in Survivin-deltaEx3: a potential link between nucleolus and protein degradation Oncogene. 2005 Apr 14;24 (16):2723-34.

(42) Yang Y, Hu W, Feng S, Ma J, Wu M. RIP3 beta and RIP3 gamma, two novel splice variants of receptor-interacting protein 3 (RIP3), downregulate RIP3-induced apoptosis. Biochem Biophys Res Commun. 2005 Jun 24;332(1):181-7.

(43) Yang Y, Ma J, Chen Y, Wu M. Nucleocytoplasmic shuttling of receptor-interacting protein 3 (RIP3): identification of novel nuclear export and import signals in RIP3. J Biol Chem. 2004 Sep 10;279 (37):38820-9.

(44) Song Z, Liu S, He H, Hoti N, Wang Y, Feng S, Wu M. A single amino acid change (Asp   53 --> Ala53) converts Survivin from anti-apoptotic to pro-apoptotic. Mol Biol Cell. 2004 Mar;15 (3):1287-96.

(45) Hoti N, Zhu DE, Song Z, Wu Z, Tabassum S, Wu M. p53-dependent apoptotic mechanism of a new designer bimetallic compound tri-phenyl tin benzimidazolethiol copper chloride (TPT-CuCl2): in vivo studies in Wistar rats as well as in vitro studies in human cervical cancer cells. J Pharmacol Exp Ther. 2004 Oct;311(1):22-33.

(46) Song Z, Yao XB, Wu M. Direct interaction between survivin and Smac/DIABLO is essential for the anti-apoptotic activity of survivin during taxol-induced apoptosis J Biol Chem. 2003 Jun 20;278 (25):23130-40.

(47) Hoti N, Ma J, Tabassum S, Wang Y, Wu M. Triphenyl tin benzimidazolethiol, a novel antitumor agent, induces mitochondrial-mediated apoptosis in human cervical cancer cells via suppression of HPV-18 encoded E6. J Biochem (Tokyo). 2003 Oct;134(4):521-8.

(48) Miao L, Yi P, Wang Y, Wu M. Etoposide upregulates Bax-enhancing tumour necrosis factor-related apoptosis inducing ligand-mediated apoptosis in the human hepatocellular carcinoma cell line QGY-7703. Eur J Biochem. 2003 Jul;270(13):2721-31.

(49) Yi P, Zhang W, Zhai Z, Miao L, Wang Y, Wu M. Bcl-rambo beta, a special splicing variant with an insertion of an Alu-like cassette, promotes etoposide- and Taxol-induced cell death. FEBS Lett. 2003 Jan 16;534(1-3):61-8.

(50) Yang Y, Ma J, Song Z, Wu M. HIV-1 TAT-mediated protein transduction and subcellular localization using novel expression vectors. FEBS Lett. 2002 Dec 4;532(1-2):36-44.

(51) Colby Geoffrey, Mian Wu, Tzagoloff Alexander. MTO1 codes for a mitochondrial protein required for respiration in paromomycin-resistant mutants of Saccharomyces cerevisiae J. Biol Chem (1998) 273, 27945-27952

(52) Wong SM, Mahtani PH, Lee KC, Yu HH, Tan Y, Neo KK, Chan Y, Wu M, Chng CG. Cymbidium mosaic potexvirus RNA: complete nucleotide sequence and phylogenetic analysis. Arch Virol. (1997) 142 (2):383-91.

(53) Tzagoloff, J. Jang, D.M.Glerum and M. Wu FLX1 codes for a carrier protein involved in maintaining a proper balance of Flavin Nucleotide in yeast mitochondria J. Biol. Chem (1996) 271 7792-7797

(54) P.H.N. Tan, S.M. Wong, M. Wu, I.D. Bedford and J. Stanley. Genome organization of ageratum yellow vein virus, a monopartite whitefly-transmitted geminivirus isolated from a common weed. J. Gen. Virology (1995), 76, 2915-2922.

(55) M. Wu, B. Repetto, M. Glerium and A. Tzagoloff. Cloning and characterization of FAD1, the structural gene for Flavin Dinucleotide Synthetase of Saccharomyces cerevisiae. Mol. Cell. Biol. (1995) 15, 264-271.

(56) M. Wu, H. S. Shang, C. Y. Yeong, P. H. N. Tan, Z. X. Lu and S. M. Wang.   A novel method employing Polymerase Chain Reaction to disrupt genes lacking convenient restriction sites in yeast. Molecular Biotechnology (1995), 3, 72-74.

(57) M. Wu, S.M. Wong, H. M. Tan and R. Ting   Single base pair change ATG - ATC nullifies the cytoplasmic fumarase activity in yeast Saccharomyces cerevisiae. Biochem and Biophys Res Comm. (1995) 275, 578-590.

(58) H. S. Shang, S. M. Wong, H. M. Tan and M. Wu. YKE2, a yeast nuclear gene encoding a protein showing homology with mouse KE2 and containing a putative leucine zipper motif. Gene (1994) 151, 197-201.

(59) M. Wu and H. M. Tan The Saccharomyces cerevisiae homologue of ribosomal protein S26. Gene (1994) 150, 401-402.

(60) M. Wu and A. Tzagoloff. Identification and characterisation of a new gene (CBP3) required for the expression of yeast coenzyme QH2-cytochrome c reductase. J. Biol. Chem. (1989) 264, 11122-11130.

(61) M.Crivellone, M. Wu and A. Tzagoloff. Analysis of structural mutants of the yeast coenzyme QH2-cytochrome c reductase complex. J. Biol. Chem. (1988) 263, 14323-14333.

(62) Tzagoloff, M. Crivellone, A. Gample, I. Muroff, M. Nishikmi and M. Wu. Mutational analysis of the yeast coenzyme QH2-cytochrome c reductase complex. Phil. Trans. R. Soc. (London) (1988) B319, 107-120.

(63) M. Wu and A. Tzagoloff. Mitochondrial and cytoplasmic fumarase in yeast Saccharomyces cerevisiae are encoded by a single gene FUM1. J. Biol. Chem. (1987) 262, 12275-12282.

(64) Tzagoloff, M. Wu and M. Crivellone. Characterisation of COR1, the structural gene for the 44-kilodalton core protein of yeast coenzyme QH2-cytochrome c reductase. J. Biol. Chem. (1986) 261, 17163-17169.

(65) J. Morin, A. Black, M. Wu and S. Beychok. cDNA of the immunoglobulin k chain of an Epstein-Barr virus-transformed human lymphoid cell line: partial sequence determination and bacterial expression. Proc. Natl. Acad. Sci. U. S. A. (1985) 82, 7025-7029.

参与撰写专著章节列表

1.《泛素介导的蛋白质降解》1版,中国协和医科大学出版社; 2008.11.01;第5章:泛素连接酶 pp163-245,吴缅等

2.《分子细胞生物学》第2版,清华大学出版社; 2011.09.01;第30章:细胞死亡的分子调控 pp535-560,吴缅等

3.《肿瘤生物学导论》科学出版社;2014.06.01;第9章:癌细胞特征之七——细胞代谢失控 pp182-206吴缅等